An Aβ3-10-KLH vaccine decreases Aβ plaques and astrocytes and microglia activation in the brain of APP/PS1 transgenic mice

This study aimed to investigate β-amyloid peptide (Aβ) plaques and changes of astroglia microglia in mice with Alzheimer’s disease (AD). In this study, 18 transgenic amyloid precursor protein (APP)/presenilin-1 (PS1) were randomized into the Aβ3-10-KLH vaccine, Aβ1-42 phosphate-buffered saline (PBS) groups. The injected at different time points. Morris water maze test was used identify spatial learning memory abilities mice. Immunohistochemistry done examine Aβ, glial fibrillary acidic protein, transmembrane 119 (TMEM119). Correspondingly, enzyme-linked immunosorbent assay (ELISA) measure interleukin (IL) -1β tumor necrosis factor (TNF) -α brain results showed that both vaccine could improve cognitive function APP/PS1 significantly. inoculations reduced Aβ load suppressed astrocytes proliferation cortex compared PBS group. While there no significant difference between two groups, vaccines decreased levels IL-1β TNF-α as group, but without vaccines. conclusion, early immunotherapy an reduces activation cells deposition plaque